2019-06-01 · However, the overexpression of ABI5 in the bes1-D background could not rescue the early-flowering phenotype . Because plants overexpressing ABI5 decreased the ABA insensitivity of bes1-D , it is indicated that ABI3-mediated negative regulation of the floral transition would be independent of ABI5 activity, at least under higher-BR-response conditions.

Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas exchange when plants experience periodic drought.

ABI5 is mainly expressed in dry seeds, and its expression markedly decreases after germination (Finkelstein and Lynch, 2000b; Lopez‐Molina et al., 2001). Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001). Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ-accumulating (JAZ-ΔJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ repressors of the JA pathway regulate the ABA-responsive ABI3 and ABI5 transcription factors to integrate JA and ABA signals during seed germination and post-germinative growth. ABI5 overexpression also results in high sensitivity to glucose and anthocyanin accumulation in response to sugar stress (Finkelstein et al., 2002).

Abi5 overexpression

Furthermore, we showed that ABI5 directly binds to the CAT1promoter and activates CAT1expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germi- nation. Se hela listan på frontiersin.org Overexpression of ABI5 Confers ABA Sensitivity to hy5 and Inhibits Hypocotyl Elongation. If ABI5 is a direct target of HY5, one would expect that overexpression of ABI5 may be able to rescue some of the hy5 mutant phenotypes such as its reduced sensitivity to ABA. The ABI5 overexpression lines were generated in both Col-0 and hy5 background 2020-04-11 · Functional analysis indicated that overexpression of GmSUMO2 gene in soybean hairy roots accentuated the sensitivity to exogenous ABA. Furthermore, the expression levels of ABI3, ABI5, SnRK1.1 and SnRK1.2 were differentially regulated by GmSUMO2 in transgenic soybean hairy roots.

It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis.

These results indicate that HY5 inte-gratesbothABAandlightsignaltransductionpathwayspartially through direct activation of ABI5. Results HY5 Mediates ABA Response in Roots and Is Required for Osmotic The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked.

Conversely, overexpression of ABI5 enhanced light‐mediated hypocotyl inhibition in seedlings (Chen et al., 2008). Intriguingly, the regulatory interactions among light signalling factors while they modulate ABA signalling can sometimes be different from their primary interactions during light‐related developmental processes.

Our study suggests that FHY3 and FAR1 are positive regulators of ABA signaling. It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. (B) Phenotypes of abi5-1, ABI5 overexpression lines (OE) and WT seeds sown on plates containing DMSO, ABA (1 μM), and AZD (1 μM) for 14 days. Supplementary Figure 3 | GUS staining of ABI5-GUS OE12 transgenic line.

ABI5 affects reactive oxygen species (ROS) homeostasis by affecting CATALASEexpression and cata- lase activity. Furthermore, we showed that ABI5 directly binds to the CAT1promoter and activates CAT1expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germi- nation. Se hela listan på frontiersin.org Overexpression of ABI5 Confers ABA Sensitivity to hy5 and Inhibits Hypocotyl Elongation.
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Overexpression of ABI5 reduces, whereas loss of function in the abi5 mutant increases, Cd accumulation in Arabidopsis, indicating the involvement of ABI5 in modulating Cd uptake. To identify transcriptional regulators involved in ABI5-mediated Cd accumulation, we searched for ABI5-interacting proteins and identified the Cd-induced R2R3-MYB TF MYB49 . Overexpression of ABI5 altered flowering time. (A) The expression level of ABI5-HA fusion protein in individual transgenic lines of p35S::ABI5-HA. Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ- accumulating (JAZ-DJas) plants.

Moreover, the expression of eight other PYLs is also affected during the germination of abi5 mutants.
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Srinivas S. L. Gampala, Ruth R. Finkelstein, Samuel S. M. Sun, Christopher D. Rock

Overexpression of MdABI5 promoted ABA-induced anthocyanin biosynthesis, which was partially dependent on MdbHLH3. Further studies showed that MdABI5 can not only interact with, and enhance the transcriptional activity of MdbHLH3 to target genes MdDFR and MdUF3GT, but can also directly bind to the promoter of MdbHLH3 and activate its expression. It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants. ABI5 was previously reported as the target protein of AFP2 during seed germination (Lopez-Molina et al., 2003), and overexpression of ABI5 activates the flowering negative factor FLC at the transcriptional level to repress flowering (Wang et al., 2013b).